Recombinant Human Anti-IAV HA Antibody (PABC-393) (CAT#: PABC-393)
This product is a recombinant human antibody that can recognize IAV HA. This antibody effectively reacting with all influenza A hemagglutinin (HA) subtypes. The antibody is the second cross-group neutralizing antibody for which structures of complexes with both group1 and group 2 HAs have been reported.
Specific Inquiry
SDS-PAGE
Figure 1 Recombinant Human Anti-IAV HA Antibody (PABC-393) in SDS-PAGE
SDS-PAGE analysis of PABC-393 in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Recombinant Human Anti-IAV HA Antibody (PABC-393) in HPLC
HPLC analysis of PABC-393 shows the purity is>90%.
WB
Figure 3 Human Anti-IAV HA Antibody (PABC-393) in WB 1
Western blot analysis was performed by loading Antigen Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-IAV HA Antibody (PABC-393) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.3 μg)
Lane 2: Reducing antigen (0.6 μg)
WB
Figure 4 Human Anti-IAV HA Antibody (PABC-393) in WB 2
Western blot analysis was performed by loading Antigen Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-IAV HA Antibody (PABC-393) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.3 μg)
Lane 2: Reducing antigen (0.6 μg)
WB
Figure 5 Human Anti-IAV HA Antibody (PABC-393) in WB 3
Western blot analysis was performed by loading Antigen Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-IAV HA Antibody (PABC-393) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.3 μg)
Lane 2: Reducing antigen (0.6 μg)
WB
Figure 6 Human Anti-IAV HA Antibody (PABC-393) in WB 4
Western blot analysis was performed by loading Antigen Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein, His Tag onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-IAV HA Antibody (PABC-393) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.3 μg)
Lane 2: Reducing antigen (0.6 μg)
ELISA
Figure 7 Human Anti-IAV HA Antibody (PABC-393) in ELISA 1
ELISA analysis of PABC-393 was performed by coating with Antigen Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with Anti-IAV HA Antibody (PABC-393) at a starting concentration of 2 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 8 Human Anti-IAV HA Antibody (PABC-393) in ELISA 2
ELISA analysis of PABC-393 was performed by coating with Antigen Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with Anti-IAV HA Antibody (PABC-393) at a starting concentration of 2 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 9 Human Anti-IAV HA Antibody (PABC-393) in ELISA 3
ELISA analysis of PABC-393 was performed by coating with Antigen Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with Anti-IAV HA Antibody (PABC-393) at a starting concentration of 2 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
ELISA
Figure 10 Human Anti-IAV HA Antibody (PABC-393) in ELISA 4
ELISA analysis of PABC-393 was performed by coating with Antigen Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein, His Tag. Then blocked with BSA and incubated with Anti-IAV HA Antibody (PABC-393) at a starting concentration of 2 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 11 Human Anti-IAV HA Antibody (PABC-393) in DB 1
Dot Blot analysis of PABC-393 was performed by coating with Antigen Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
PABC-393 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
DB
Figure 12 Human Anti-IAV HA Antibody (PABC-393) in DB 2
Dot Blot analysis of PABC-393 was performed by coating with Antigen Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
PABC-393 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
DB
Figure 13 Human Anti-IAV HA Antibody (PABC-393) in DB 3
Dot Blot analysis of PABC-393 was performed by coating with Antigen Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
PABC-393 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
DB
Figure 14 Human Anti-IAV HA Antibody (PABC-393) in DB 4
Dot Blot analysis of PABC-393 was performed by coating with Antigen Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein, His Tag.
PABC-393 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
Specifications
- Host Species
- Human
- Type
- Human IgG, κ
- Specificity
- IAV HA
- Species Reactivity
- IAV
- Applications
- Neut, FuncS, ELISA
- Related Disease
- Infectious disease
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Antibody MEDI8852 has been reported in the application of Neutralization, Functional Assay and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- IAV HA; Influenza A Virus Hemagglutinin; Hemagglutinin HA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
| NABG-078 | Recombinant Anti-IAV HA VHH Single Domain Antibody | FC, CA, IP, FuncS | Llama VHH |
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| HPAB-0345CQ | Recombinant Llama Anti-IAV HA Single Domain Antibody (HPAB-0345CQ) | ELISA, Neut | Llama VHH |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-393, RRID: AB_3111586)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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